Table 1.
Thermodynamic parameters obtained from analysis of ITC data for CbpA fragments binding to SC-D15 in solution
| Construct | ΔG (kcal mol−1) | ΔH (kcal mol−1) | −T ΔS (kcal mol−1) | Nnormalizeda (moles of CbpA per moles of SC) |
|---|---|---|---|---|
| CbpA-R1 | −9.82±0.01 | −26.60±0.05 | +16.78±0.10 | 1.86±0.01 |
| CbpA-R2 | −10.23±0.02 | −21.19±0.07 | +10.96±0.10 | 2.19±0.01 |
| CbpA-R12 | −10.39±0.02 | −46.30±0.14 | +35.91±0.10 | 0.87±0.01 |
| CbpA-NR12 |
−10.40±0.01 |
−38.88±0.07 |
+28.48±0.10 |
1.07±0.01 |
| aNnormalized values were determined using 1.42 μM as the effective SC-D15 protein concentration (see Materials and methods for details). ΔG, ΔH and −T ΔS values are in units of kcal per mole of CbpA construct. | ||||