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. 2004 Nov 25;24(1):138–148. doi: 10.1038/sj.emboj.7600491

Figure 4.

Figure 4

Analysis of loop mutants of human pri-miR-27a and pri-miR-31. (A) miR-27a expression. Plasmids encoding wild-type pri-miR-27a, or one of two mutants (1 and 2), were cotransfected with pH1-GFP into 293T cells. Northern blotting was performed to detect the expression of pre- and mature miR-27a (upper panel) and the control GFP siRNA (lower panel). (B) miR-31 expression. Three pri-miR-31 mutants were constructed (boxed sequences), and their miRNA expression levels compared to wild-type miR-31 in transfected 293T cells.