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. 2004 Nov 25;24(1):138–148. doi: 10.1038/sj.emboj.7600491

Figure 7.

Figure 7

Movement of the loop/stem junction affects Drosha cleavage site selection. (A) Schematic of the predicted RNA secondary structures adopted by the J+1 and J−1 mutants of pCMV-miR-30a(E10). Mutated residues are underlined and the arrows indicate the 5′ ends of the miRNAs, as defined in the primer extension shown in the lower panel. Sequences of the size standards used are given in Supplementary Table 1. (B) Drosha processing of miR-30a transcripts in vitro. M: DNA markers. Pre-miRNA bands are indicated by asterisks. (C) To test the identities of Drosha cleavage products, RNAs from bands A, B, and C in panel B were isolated, digested with recombinant human Dicer, and resolved on a 15% denaturing gel.