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. 2015 Dec 9;14(5):514–521. doi: 10.1080/15476286.2015.1122162

Figure 2.

Figure 2.

The application of zebrafish in drug screening CiRS-7 is associated with impaired midbrain development in zebrafish and can reduce the biological function of miR-7, leading to oncogenesis and a series of malignant behaviors of alimentary cancer. Therefore, drugs that can downregulate ciRS-7 would be promising for the treatment of cancer. The size of the zebrafish midbrain in group A was achieved when cultured with no other influencing factors applied and the size is recorded as the control. In group B, plasmid DNA producing circular ciRS-7 was induced in the culture of zebrafish embryos and the size of the midbrain is smaller than normal, suggesting impaired midbrain development. Upon injecting placebo and plasmid DNA producing circular ciRS-7 in group C, the size of the zebrafish midbrain remained impaired. By applying a drug and plasmid DNA producing circular ciRS-7 in group D, the impaired midbrain development recovered to the normal size, suggesting that the drug is effective in downregulating the function of ciRS-7 and may be useful to treat cancer.