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. 2005 Jan 6;24(1):149–159. doi: 10.1038/sj.emboj.7600498

Figure 2.

Figure 2

Lipid specificity of the farnesol effect. (A) Effects of the similarly sized alcohols tetradecanol (14-OH) and hexadecanol (16-OH). Microsomes bearing Hmg2p-GFP were resuspended in buffer with DMSO, 200 μM FOH, 200 μM 14-OH, or 200 μM 16-OH, and then incubated with trypsin (15 μg/ml) at 30°C. Samples were removed at the indicated times and evaluated by 14% SDS–PAGE and immunoblotting of the Myc epitope. (B) Effects of related isoprenoids geraniol (GOH) and geranylgeraniol (GGOH). Evaluation was as described in panel A. (C) Effects of the farnesol isomer nerolidol (NOH) and of saturated farnesol (TMD). Evaluation was as described in panel A. (D) Structures of the compounds tested. (E) Effects of the yeast sterols ergosterol (erg) and lanosterol (lan). Evaluation was as described in panel A.