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. 2004 Dec 16;24(1):85–96. doi: 10.1038/sj.emboj.7600500

Figure 1.

Figure 1

Impaired NF-κB-dependent gene expression in Carm1(−/−) cells in response to proinflammatory stimuli. (A) Impaired expression of MIP-2, MCP-1, G-CSF, ICAM1 and IP-10 in Carm1(−/−) MEF cells in response to LPS. Carm1(+/+) and Carm1(−/−) MEF cells were treated with LPS (10 μg/ml) and RNA isolated at the indicated time points, followed by RT–PCR determination of MIP-2, MCP-1, G-CSF, ICAM1, IP-10, IL-6, KC, COX-2, HPRT and β-actin mRNA. (B, C) Impaired NF-κB-dependent gene expression in Carm1(−/−) cells in response to TNFα and LPS. Carm1(+/+) MEF cells (B) and Carm1(−/−) MEF cells (C) were cotransfected with HIV-luc or HIVmut-κB-luc (2 μg) and pphRSVnt-β-gal (200 ng) together with CMV-CARM1 (500 ng) or CMV empty vector as indicated. Cells were subsequently stimulated with TNFα (10 ng/ml) or LPS (10 μg/ml) 24 h after transfection for 8 h. The indicated activation was determined by the ratio of the relative luciferase activity measured for the HIV-luc (black bars) or HIVmut-κB-luc (white bars) reporter gene after stimulation. The ratio obtained for untreated cells was arbitrarily set to 1. Error bars indicate s.e. of three independent experiments.