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. 2004 Dec 16;24(1):85–96. doi: 10.1038/sj.emboj.7600500

Figure 4.

Figure 4

CARM1 synergistically coactivates the NF-κB-mediated transactivation, in concert with the transcriptional coactivator p300/CBP. (A, B) CARM1 synergistically activates together with p300 NF-κB-dependent gene expression in response to proinflammatory stimuli. Carm1(+/+) MEF cells (left panel) and Carm1(−/−) MEF cells (right panel) were cotransfected with HIV-luc or HIVmut-κB-luc (2 μg) and pphRSVnt-β-gal (200 ng), together with CMV-CARM1 (200 ng) and RSV-p300 (500 ng) or CMV and RSV empty vectors as indicated. Cells were subsequently stimulated with TNFα (10 ng/ml) (A) or LPS (10 μg/ml) (B) 24 h after transfection for 8 h. Cells were harvested 32 h after transfection and the indicated activation of NF-κB-dependent gene expression determined as described in Figure 1. (C) CARM1 synergistically activates together with p300 and p65-dependent gene expression. Carm1(+/+) MEF cells (left panel) and Carm1(−/−) MEF cells (right panel) were cotransfected with HIV-luc or HIVmut-κB-luc (2 μg) and pphRSVnt-β-gal (200 ng) together with CMV-p65 (80 ng) CMV-CARM1 (200 ng) and RSV-p300 (500 ng) or CMV and RSV empty vectors as indicated. The indicated activation of NF-κB-dependent gene expression was determined as described in Figure 1.