FIGURE 1:

(A) Mature hippocampal neurons were immunostained with anti-Puralpha and anti–PSD-95 antibodies. White arrows indicate the colocalization of Puralpha and PSD-95 clusters. (B) Neurons were transfected with TagRFP and immunostained with anti-Puralpha antibody. Note that some Puralpha clusters are localized in dendritic spines (arrows). (C) Neurons were immunostained with anti-Stau1 and anti–PSD-95. (D) Neurons were transfected with TagRFP and immunostained with anti-Stau1 antibody. (E) Quantification. Error bars indicate SEM; 10–15 cells from three mice were examined. (F) Mature hippocampal neurons were transfected with YFP-Puralpha and immunostained with anti–PSD-95. White arrows indicate the colocalization of Puralpha and PSD-95 clusters. (G) TagRFP was coexpressed with YFP-Puralpha. Some Puralpha clusters localize to dendritic spines (arrows). (H) Neurons were transfected with Stau1-GFP and were subsequently immunostained with anti–PSD-95. (I) TagRFP is coexpressed with Stau1-GFP. (J) Quantification. Error bars indicate SEM; 20–27 cells from three mice were examined. ***, p < 0.001; Student’s t test. Scale bars: 5 μm.