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. 2017 Jun 1;28(11):1418–1425. doi: 10.1091/mbc.E16-08-0571

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© 2017 Semenova et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Pigment aggregation signals do not stimulate MT nucleation on the centrosome. Fluorescence images of melanophores expressing EGFP-EB1 injected with either control nonimmune IgG (top) or TYRP1 antibodies (bottom) treated with MSH (left) or melatonin (right); circles (D = 12 μm) outline the areas used for counting EGFP-EB1 comets; in the control cell, which aggregated pigment granules, melatonin treatment induced a significant increase in the number of EGFP-EB1 comets in the centrosome area, whereas in the TYRP1 antibody–injected cell, which did not accumulate pigment granules in the cell center, the number of comets surrounding the centrosome did not change significantly after stimulation with melatonin; bar, 10 μm.