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. 2017 Jun 1;28(11):1489–1506. doi: 10.1091/mbc.E17-03-0195

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© 2017 Lu, Acoba, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — ANTs assemble in high–molecular weight complexes. Mitochondria (200 μg) isolated from HEK293 Flp-In cells (wt) expressing endogenous ANT2/3 (A) or overexpressing (B, C) the indicated ANT constructs or from the indicated mouse tissues were solubilized with digitonin, resolved by 2D BN/SDS–PAGE, and immunoblotted for ANTs using (A, B) ANT2/ANT3, (C) ANT1, or (B) FLAG antibodies. UQCRC2, NDUFB6, and COX4 are presented to show ANT comigration with RSCs. In B, overexpressed CNAP-ANT2 and endogenous ANT2/3 are indicated.