FIGURE 6:
ant2TALEN cells have altered bioenergetics parameters, reduced respiration, and increased sensitivity to ANT inhibitor. (A) OCR (pmol O2/min ± SEM; n ≥ 40) were measured using a Seahorse XF96e FluxAnalyzer with the Mito Stress Test kit under indicated conditions. (B) Basal and maximal OCR were obtained under glucose stimulation or after FCCP treatment to uncouple mitochondria. ATP production is expressed as basal OCR subtracted by postoligomycin oxygen consumption. Proton-leak OCR details oxygen consumed by the mitochondrion after oligomycin treatment. Degree of coupling (percentage) and spare respiratory capacity (as percentage of wt) obtained under glucose stimulation or after FCCP treatment to uncouple mitochondria. Data represent mean ± SEM. n ≥ 79. (C) Basal and maximal OCRs, ATP production, proton-leak OCR, degree of coupling, and spare respiratory capacity of wt and ant2TALEN cells after overnight incubation with the indicated concentrations of BKA. Data represent mean ± SEM. n ≥ 79.