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. 2017 May 23;10:2701–2709. doi: 10.2147/OTT.S114318

Figure 5.

Figure 5

PTEN is a direct target of miR-216a in OC cells.

Notes: (A) MiR-216a and its complementary sequence in the 3′-UTR of PTEN. (B) Overexpression of miR-216a significantly inhibited the luciferase activity that carried wt 3′-UTR of PTEN but had no obvious influence on mt 3′-UTR of PTEN in CAOV3 cells. **P<0.01. (C) Inhibition of miR-216a obviously increased the luciferase activity that carried wt 3′-UTR of PTEN but had no obvious influence on mt 3′-UTR of PTEN in SKOV-3 cells. **P<0.01. (D) Overexpression of miR-216a significantly decreased PTEN mRNA level in CAOV3 cells. **P<0.01. (E) Inhibition of miR-216a significantly increased PTEN mRNA level in SKOV-3 cells. **P<0.01. (F) Overexpression of miR-216a significantly inhibited the expression of PTEN and increased p-AKT in CAOV3 cells. *P<0.05, **P<0.01. (G) Inhibition of miR-216a significantly increased the expression of PTEN and decreased p-AKT in SKOV-3 cells. *P<0.05, **P<0.01.

Abbreviations: OC, ovarian cancer; PTEN, phosphatase and tensin homolog; wt, wild type; mRNA, messenger RNA; NC, negative control; mt, mutant.