Figure 9. Relative hydrophobicity of TMD5 coupled with C-terminal positive charges modulate interaction of Sco3746 with the Sec pathway.

(A) The sequence flanking TMD5 of Sco3746. The lower sequence extends to the position of the ‘short’ Sco3746-Bla fusion, and the amino acids in TMD5 substituted for leucine in this construct are shown. Top is the sequence fused to Bla in the ‘long fusion’. The predicted position of TMD5 was determined using the SCAMPI2/TOPCONS servers (Tsirigos et al., 2015, 2016) and is shown boxed. The twin arginines are shown in purple and positively charged amino acids C-terminal to TMD5 are underlined. (B) Representative images of M.I.C.Evaluator strip tests of strain DADE (tat^-) harbouring pSU18 producing the indicated variants of Sco3746_TMD-Bla In each panel the mean M.I.C ± s.d. is given at the bottom of each test strip (where n = 3 biological replicates for each strain).

DOI: http://dx.doi.org/10.7554/eLife.26577.023