Table 2.

Effect of amino acid truncation in the Sco2149 cytoplasmic loop region on the ability of Sco2149_TMD-AmiA and Sco2149_TMD-Bla to support growth on SDS or ampicillin, respectively. Note that growth on ampicillin was scored using the tat^- strain DADE and therefore assesses Sec translocation only. Y indicates growth on 1% SDS, N indicates no growth. Mean M.I.C for growth on ampicillin is given in µg/ml + one standard deviation, n = at least 3.

DOI: http://dx.doi.org/10.7554/eLife.26577.009

Variant	Growth on 1% SDS (Tat translocation)	Mean M.I.C. for ampicillin (Sec translocation)
wild type	Y	7.6 ± 0.9
5 residue truncations
∆118–122	Y	10.0 ± 2.3
∆123–127	Y	5.3 ± 2.3
∆128–132	Y	12.0 ± 0.0
∆133–137	Y	6.7 ± 2.3
∆138–142	Y	7.0 ± 2.0
∆143–147	Y	6.7 ± 2.3
∆148–152	Y	10.0 ± 3.3
∆153–157	Y	9.6 ± 3.6
10 residue truncations
∆118–127	Y	6.0 ± 0.0
∆128–137	Y	9.5 ± 3.0
∆138–147	Y	8.0 ± 0.0
∆148–157	Y	9.3 ± 2.3
15 residue truncations
∆118–132	Y	9.0 ± 3.8
∆123–137	Y	8.0 ± 0.0
∆128–142	Y	10.0 ± 2.3
∆133–147	Y	9.3 ± 2.3
∆138–152	Y	5.0 ± 1.4
∆143–157	Y	4.8 ± 1.5
20 residue truncations
∆118–137	Y	7.3 ± 1.2
∆138–157	Y	5.5 ± 1.9
25 residue truncations
∆118–142	Y	12.0 ± 0.0
∆123–147	Y	7.6 ± 0.9
∆128–152	Y	8.0 ± 0.0
∆133–157	Y	6.0 ± 0.0
30 residue truncations
∆118–147	Y	10.0 ± 2.8
∆123–152	Y	13.6 ± 2.2
∆128–157	Y	7.3 ± 1.2
>35 residue truncations
∆118–152	Y	12.0 ± 0.0
∆123–157	N	3.0 ± 0.0
∆118–153	Y	16.0 ± 0.0
∆118–154	Y/N	9.3 ± 2.3
∆118–155	N	4.0 ± 0.0
∆118–156	N	4.0 ± 0.0
∆118–157	N	2.5 ± 0.6