Table 5.
Predicted △Gapp values (in kcal mol−1) for membrane insertion of the first and last TMDs of the indicated predicted polyferredoxin proteins. Sequences were analysed using the △Gapp prediction server (http://dgpred.cbr.su.se/) that are based on hydrophobicity scales generated from (Hessa et al., 2005, 2007). This server uses the SCAMPI2/TOPCONS servers (Tsirigos et al., 2015, 2016) to predict the positions of the TMDs and for delta proteobacterium MLMS-1 Q1NSB0 (PFD) predicts TMD1 to span aa 9–31 and TMD5 to span aa 338–359.
| Predicted △Gapp | |||
|---|---|---|---|
| Family/Species | Protein ID | TM1 | TM5* |
| Latescibacteria bacterium DG_63 | A0A0S7XC05 | −1.792 | 0.371 |
| Nitrospirae bacterium GWA2_46_11 | OGW21092.1 | −1.062 | 1.222 |
| Caldithrix sp. RBG_13_44_9 | OGB67066.1 | −3.560 | 1.438 |
| Thermoplasmatales archaeon DG-70–1 | KYK31751.1 | −1.474 | 1.715 |
| Anaerolinea thermolimosa | WP_062193756.1 | −2.317 | 1.107 |
| Anaeroarcus burkinensis | WP_027938385.1 | −0.975 | 0.836 |
| delta proteobacterium MLMS-1 | Q1NSB0 | −2.382 | 0.297 |
| delta proteobacterium MLMS-1 G354L, R358L | −0.701 | ||
| delta proteobacterium MLMS-1 G354L, P355L, R358L | −1.741 | ||
*Bla is fused to delta proteobacterium MLMS-1 PFD (Q1NSB0) after amino acid 364 (full sequence of all of the fusion proteins used in this study can be found in Supplementary file 1D).