. Author manuscript; available in PMC: 2017 May 30.

Published in final edited form as: Nat Plants. 2016 Nov 28;2:16183. doi: 10.1038/nplants.2016.183

Table 1. Abbreviation and functional summary of the cucurbitacin biosynthetic and regulatory genes in three cucurbits.

Orthologous genes are listed in row and indicated with the same background. The co-expressed genes encoding potential CuC, CuB or CuE biosynthetic enzymes or regulators are indicated in bold. C2, C11, C20, C19, and C25 represent the corresponding carbon atom of cucurbitadienol. The evolutionary relationship of the genes encoding CYP88s or bHLH TFs is shown in Supplementary Figure 1.

Gene type	Cucumis sativus			Cucumis melo			Citrullus lanatus
Gene type	Gene ID	Abbr.	Remark	Gene ID	Abbr.	Remark	Gene ID	Abbr.	Remark
Biosynthetic genes

CYP81Q58	*Csa6G088160*	*Cs160*	C25 hydroxylase	*Melo3C022377*	*Cm160*		*Cla007077*	*Cl160*

CYP89A140	*Csa6G088170*	*Cs170*		*Melo3C022376*	*Cm170*		*Cla007078*	*Cl170*

CYP81Q59	Csa6G088180	Cs180		*Melo3C022375*	*Cm180*	C2 hydroxylase	*Cla007079*	*Cl180*	C2 hydroxylase

OSC	*Csa6G088690*	*CsBi*	cucurbitadienol synthase	*Melo3C022374*	*CmBi*	cucurbitadienol synthase	*Cla007080*	*ClBi*	cucurbitadienol synthase

ACT	*Csa6G088700*	*CsACT*	acetyltransferase	*Melo3C022373*	*CmACT*	acetyltransferase	*Cla007081*	*ClACT*	acetyltransferase

CYP87D19	Csa6G088710	Cs710		*Melo3C022372*	*Cm710*		*Cla007082*	*Cl710*

CYP87D20	*Csa1G044890*	*Cs890*	C11 carbonylase + C20 hydroxylase	*Melo3C002192*	*Cm890*	C11 carbonylase + C20 hydroxylase	*Cla008355*	*Cl890A*	C11 carbonylase + C20 hydroxylase
CYP87D20	*Csa1G044890*	*Cs890*	C11 carbonylase + C20 hydroxylase	*Melo3C002192*	*Cm890*	C11 carbonylase + C20 hydroxylase	*Cla008354*	*Cl890B*	C11 carbonylase + C20 hydroxylase

CYP712D8	*Csa3G698490*	*Cs490*		*Melo3C023960*	*Cm490*		*Cla017252*	*Cl490*

CYP88A60	Csa3G903510	Cs510		Melo3C003387	Cm510		*Cla016164*	*Cl510*

CYP88L2	Csa3G903530	Cs530		Predicted^T	Cm540
	*Csa3G903540*	*Cs540*	C19 hydroxylase

CYP88L3	*Csa3G903550*	*Cs550*		Melo3C003385^T	Cm550A
				Melo3C003384^T	Cm550B

CYP88L4	Csa3G903560	Cs560		Melo3C003383^T	Cm560A		Predicted	Cl560A
				Predicted^Δ	Cm560B		Cla016162	Cl560B

Regulatory genes

bHLH TF							Predicted	C1507

	Predicted^T	Cs508		Predicted^T	Cm508		*Cla011508*	*ClBt*	fruit specific

	*Csa5G156220*	*CsBl*	leaf specific	Melo3C005612	Cm612
				*Melo3C005611*	*CmBt*	fruit specific

	Csa5G157220	Cs220		*Melo3C005610*	*CmBr*	root specific	Predicted	C1509

	*Csa5G157230*	*CsBt*	fruit specific	Predicted^Δ	Cm610		*Cla011510*	*ClBr*	root specific

							Predicted^Δ	Cl511

Abbr., abbreviation; Predicted, genes not annotated in the genome database; superscript ^T, truncated gene; superscript ^Δ, premature translational termination.