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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Free Radic Biol Med. 2016 Nov 9;102:217–228. doi: 10.1016/j.freeradbiomed.2016.11.008

Fig. 3.

Fig. 3

Enhancing pp60Src activity in the mouse lung induces eNOS uncoupling. Mice were injected with either DST-luciferase (DST) or pAD/CMV/V5-DEST-CApp60Src (Y527FSrc) plasmids via a tail vein injection. After 72 h, the animals were sacrificed and peripheral lung lysates prepared. Mice injected with Y527FSrc had a significant increase in pp60Src protein levels (A) and increase in pp60Src activity, as determined by increase in phospho-Y416 Src (B). The increase in pp60Src activity resulted in an increase in NO generation (C) and p-Ser1177 eNOS levels (D). Over-expression of Y527FSrc in the mouse lung also significantly increased the levels NOS-derived superoxide (E), peroxynitrite (F), and 3-NT protein (G). Values are mean ± SEM, n=4–16, *P<0.05 vs. DST.