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The molecular nature of the e(y)3u1 mutation. (A, B) The presence of SAYP in different strains of flies revealed by Western blotting with affinity-purified Ab1 (A) or Ab2 (B). The total protein from five embryos of Oregon R (WT), e(y)3u1/e(y)3u1 (U1), e(y)3/e(y)3EMSl (L/WT) strains and transgenic e(y)3EMSl/e(y)3EMSl; P{e(y)3ΔPHD} (L/ΔP) strain was resolved in 8% SDS–PAGE. The molecular weight is indicated in kDa.
