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The conserved domain of SAYP activates reporter genes HIS3 and LacZ in yeast
| LexA fusion | Growth without histidinea | β-Galactosidase activity (U)b |
|---|---|---|
| LexA-SAYP(1273–1629) | ++ | 31±2 |
| LexA-SAYP(1366–1629) | + | 0.4±0.1 |
| LexA-SAYP(1273–1493) | − | — |
| LexA-GAL4 activation domain | ++ | 317±30 |
| a‘++' potent growth, ‘+' weak growth, ‘−' no growth. | ||
| bβ-Galactosidase activity (U) was determined using the following formula: U=1000 × OD578/(t × 0.5 × OD600), where t is incubation time (in minutes). | ||
