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. Author manuscript; available in PMC: 2017 Jun 19.
Published in final edited form as: Immunol Cell Biol. 2016 Dec 19;95(5):454–460. doi: 10.1038/icb.2016.122

Figure 3.

Figure 3

Vaccination induced antigen specific IFN-γ gene expression. Guinea pigs (n=3 per group) were euthanized one day before challenge and splenocytes were stimulated with 0.5µg of rCPAF or UV-inactivated CT-D (1×105 IFUs), or unstimulated (media alone), for 24 hrs. IFN-γ gene expression was then measured by qRT-PCR analysis and normalized to respective GAPDH expression for each sample. Subsequently, within each vaccination group, IFN-γ expression by rCPAF and CT-D (UV) stimulation was calculated and presented as a relative value to medium mock stimulation. Significant increase in IFN-γ expression between stimuli was indicated * p < 0.05, ** p < 0.01 (One-Way ANOVA). Results are representative of two independent experiments.