Table 1.

Representative examples of angiogenesis modulation via non-viral nanoparticle-based gene delivery

Angiogenesis	Disease	Factor	Delivery Vector	Animal Model	Injection	Result	Reference
Pro	Ischemic Limb	VEGF165	Heparin-PEI	Mice	Intramuscular	Over 3-fold ↑ neovasulazation vs. PEI in ischemic limb	[116]
		VEGF	PBAE	Mice	exwVoHUVEC transfection	Limb salvage 50% compared to lipoplex (30%) and PEI (12.5%)	[140]
		eNOS	Elastin-based polymer	Mice	Intramuscular	~ 3-fold ↑ perfusion ratio and blood vessel density vs. control	[120]
	Myocardial In fact	Adreno- medulliln	Dextran	Rats	ex vivo MSC transfection	2.5-fold ↑ capillary density and 40% ↓ infarct size vs. control	[131]
		VEGF165	Chitosan-g-PEI	Rats	Intramyocardial	1.6-fold ↑ejection fraction and vessel density vs. PEI control	[117]
		Hypoxia- inducible VEGF165	Cholesterol chloroformate-bPEI	Rabbits	Intramyocardial	Myocardial infarct size (36% ↓) vs. vector alone without hypoxia- inducible VEGF165	[119]
		VEGF165	PLGA	Rabbits	Intramyocardial	Capillary density (38% ↑) vs. plasmid only	[124]
	Tissue Engineering	SHh	PBAE	Mice	Transdermal	15% ↑ wound closure than PBS	[150]
		VEGF	PBAE	Mice	ex vivo ASC transfection	20% ↑ wound closure than non- modified ASC	[151]
		PDGF-B	PEI	Rats	collagen scaffold with PEI-pPDGF	40% bone volume and 52-fold ↑ connective density vs. control	[154]
Anti	Cancer	sFlt-1 or siVEGFRI	bPEI-g-PEG-cRGD	Mice	Intravenous	3-fold / 42% ↓ colon carcinoma umor growth vs. PEI-g-PEG	[214,216]
		NF-kB	Tween-SS-bPEI	Mice	Intravenous	Breast tumor volume 6% of saline	[218]
		Endostatin	PVP	Mice	Intramuscular	60% ↓ metastatic brain tumor growth vs. control by MRI	[220]
		shVEGF/ DOX	CPP-dendrimer PLL	Mice	Intravenous	VEGF mRNA (65% ↓) and median survival (2-fold ↑) vs. control in orthotopic glioma	[224]
		siVEGF	guanidine-βCD-PEG- anisamide	Mice	Intravenous	VEGF mRNA(3.5-fold ↓) / prostate tumor growth(3-fold ↓) vs. control	[228]
	Age-related Macular Degenera tion	Vasostatin	PVP	Rats	Intramuscular	32.5% / 48% decrease in CNV incidence and area at day 42	[247]
		shHIF-1α	PLGA	Rats	Intravitreal	Leakage (50%↓) / lesion thickness (40%↓) vs. control	[248]
		Flt23k	RGD-PLGA	Monkey/ Mice	Intravenous	CNV area 53% (monkey) and 43% (mice) ↓ vs. sham	[252]