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. 2017 May 30;7:2493. doi: 10.1038/s41598-017-02370-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SV analysis at high Aβ42 concentrations. Measured data overlaid with fit data and corresponding residuals are shown for 32 μM (a), 95 μM (b) and 160 μM (c) Aβ42. Below, c(s) analysis reveals the existence of a high fraction of larger oligomeric species aside from the monomer and a comparably low fraction of the 2 to 3 S species for 32 µM (d), 95 µM (e) and 160 µM (f) Aβ42. Sedimentation was performed at 60,000 rpm, 10 °C and monitored with absorbance detection at 280 nm. Normalization was performed according to peak height of the monomeric Aβ42. The insets show the distributions with an expanded y scale.