Skip to main content
. 2017 May 31;9:10. doi: 10.3389/fnsyn.2017.00010

FIGURE 2.

FIGURE 2

Prenatal DEP exposure alters microglial morphology and cortical volume in the P30 juvenile brain. (A) The two primary microglial morphological states in the P30 brain. Representative pictures of microglia with thick, long processes (left) and thin, long processes (right) in the mouse parietal cortex on P30. Photos were taken of Iba1-labeled microglia at 100X. (B) DEP males possess a significantly smaller PCX volume at P30 than VEH males, whereas females do not differ. (C) DEP/LPS males have significantly more thick, long microglia in the PCX at P30 than do VEH/LPS males, whereas females do not differ. (D) No significant differences in DG volume were detected. (E) LPS males had significantly more thick, long microglia in the DG at P30 than SAL males. DEP/LPS females had significantly more thick, long microglia than VEH/LPS and DEP/SAL females. (F) LPS males tended to have fewer thin, long microglia than SAL males in the P30 DG, whereas DEP/LPS females had significantly fewer thin, long microglia than VEH/LPS and DEP/SAL females. (G) DEP males have thin, long microglia with larger soma volumes in the DG at P30 than do VEH males, whereas females do not differ. Data are mean ± SEM, n = 5–7/group/sex. ∗∗p = 0.05, DEP vs. VEH; ∗p < 0.05 vs. VEH/LPS or DEP/SAL; ##p < 0.05, LPS vs. SAL.