Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 31;7:225. doi: 10.3389/fcimb.2017.00225

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Adams, Flores Avile and Jewett.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Dual luciferase assay with in vitro grown B. burgdorferi. Spirochetes were grown to either mid-logarithmic or stationary phase and the in vitro luciferase assay performed with 700 μM D-luciferin or 3.5 mM coelenterazine. All data are presented as mean normalized relative luciferase units ± standard deviation for biological triplicate samples. (A) Relative Rluc_Bb units normalized to OD₆₀₀. The data set for each growth condition was analyzed with a one-way ANOVA followed by Dunnett's multiple comparison test compared to B. burgdorferi expressing flaBp-rluc_Bb (+pCFA701) and Bonferroni's multiple comparison test to compare the same clone in the two growth phases. (B) Relative Fluc_Bb units normalized to OD₆₀₀ or 10⁸ relative Rluc_Bb units of the same sample. Each data set was square root transformed and analyzed with a one-way ANOVA followed by Dunnett's multiple comparison test compared to B. burgdorferi expressing flaBp-rluc_Bb (+pCFA701). Unless indicated, means were not significantly different from the control. Significant differences are indicated with asterisks (^*p ≤ 0.05; ^**p ≤ 0.01; ^***p ≤ 0.001; ^****p ≤ 0.0001).