Table 1.
Bacterial strains and plasmids used in this study.
| Strains and plasmids | Relevant features | References or source |
|---|---|---|
| STRAINS | ||
| B. breve | ||
| UCC2003 | Isolate from nursling stool | Law et al., 1995 |
| UCC2003-bfeU | pORI19-tetW-bfeU insertion mutant of UCC2003 | Christiaen et al., 2014 |
| UCC2003-sifA | pORI19-tetW-sifA insertion mutant of UCC2003 | Christiaen et al., 2014 |
| UCC2003-pK1 | UCC2003 harboring pPKCM | O'Connell Motherway et al., 2011 |
| E. coli | ||
| XL1 BLUE | supE44 hsdR17 recA1 gyrA96 thi relA1 lac F = [proAB laclq lacZ M15 Tn10(Tetr)] | Stratagene |
| EC101 | Cloning host; repA+ kmr | Law et al., 1995 |
| L. lactis NZ9000 | MG1363, pepN::nisRK, nisin-inducible overexpression host | de Ruyter et al., 1996 |
| PLASMIDS | ||
| pORI19 | Emr, repA−, ori+, cloning vector | Law et al., 1995 |
| pORI19-bfeU | pOR19 harboring internal fragment of bfeU (Bbr_0221) | Christiaen et al., 2014 |
| pORI19-bfeU-tet | pORI19 harboring internal fragment of Bbr_0221 + Tetr | Christiaen et al., 2014 |
| pORI19-sifA | pOR19 harboring internal fragment of bfeB (Bbr_0223) | Christiaen et al., 2014 |
| pORI19-sifA-tet | pORI19 harboring internal fragment of bfeB + Tetr | Christiaen et al., 2014 |
| pBC1.2 | pBC1-pSC101-Cmr | Alvarez-Martin et al., 2007 |
| pBC1.2-bfeU-IR | pBC1.2 harboring bfeU promoter | This study |
| pBC1.2-sifA-IR | pBC1.2 harboring sifA promoter | This study |
| pNZ272 | Cmr, pSH71 derivative containing promoterless glucuronidase gene for promoter screening | Platteeuw et al., 1994 |
| pNZ272-bfeU-IR | pNZ272 derivative carrying the bfeU promoter | This study |
| pNZ272-sifA-IR | pNZ272 derivative carrying the sifA promoter | This study |