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(A) Western blot analysis of sirtuin 1 (SIRT1) and β-actin (used as loading control protein) in lung cytosol and nuclear extracts. Image analyses of SIRT1 expression as determined by densitometry (B) in the cytosol and (C) in the nucleus. Data are expressed as mean ± SEM of four to six animals for each group and are expressed as ratio of relative intensity units. *P < 0.05 versus age-matched control mice; ‡P < 0.05 versus young group.
