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. 2017 May 31;8:608. doi: 10.3389/fimmu.2017.00608

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Copyright © 2017 de Haan, Reiding, Krištić, Hipgrave Ederveen, Lauc and Wuhrer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic representation of the study design. The immunoglobulin G (IgG) glycopeptides of 40 individual mice were analyzed by nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)). The mice originated from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster. Of each strain, five male and five female mice were included. Based on these analyses, relative quantification of the glycoforms was performed in a strain- and subclass-specific way. In addition, released IgG glycans of four pooled plasma samples (one male and one female) of the same strains were analyzed by nanoLC-MS(/MS), matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF(/TOF))-MS(/MS) and ultraperformance liquid chromatography (UPLC)-fluorescence for further structural elucidation of the glycoforms.