Table 1.

Detected glycoforms per IgG subclass by nanoLC-MS.

Glycoform compositiona	Depictionb	IgG1c	IgG1i	IgG2b	IgG2a/c	IgG3
H2N3F1		x	x	x	x
H5N2		x	x			x
H3N3F1		x	x	x	x	x
H3N4		x	x			x
H6N2		x
H4N3F1		x	x	x	x	x
H3N4F1		x	x	x	x	x
H4N4		x	x	x	x	x
H5N3F1			x	x	x
H4N4F1		x	x	x	x	x
H5N4		x		x	x	x
H3N5F1		x	x		x
H4N5						x
H4N3F1G1		x	x	x	x	x
H4N4G1			x
H5N4F1		x	x	x	x	x
H4N5F1		x		x	x	x
H5N3F1G1		x	x	x	x
H4N4F1G1		x	x	x	x	x
H5N4G1		x	x	x	x	x
H6N4F1		x	x	x	x	x
H5N5F1		x				x
H6N3F1G1		x	x	x	x	x
H5N4F1G1		x	x	x	x	x
H6N4G1		x
H4N5F1G1		x	x	x	x	x
H5N4G2				x	x
H6N4F1G1		x	x	x	x	x
H5N5F1G1		x		x	x
H5N4F1G1S1				x	x
H5N4F1G2		x	x	x	x	x
H5N5F1G2		x		x	x

^aH: hexose, N: N-acetylhexosamine, F: fucose, G: N-glycolylneuraminic acid, S: N-acetylneuraminic acid.

^bSymbols used: green circle: mannose, yellow circle: galactose, blue square: N-acetylglucosamine, red triangle: fucose, white diamond: N-glycolylneuraminic acid, pink diamond: N-acetylneuraminic acid. The proposed glycan structures are based on fragmentation and literature (17, 23, 35, 36). See also Table S9 in Supplementary Material.

^cPeptide sequence of IgG1: EEQFNSTFR, IgG1i: EEQINSTFR, IgG2b: EDYNSTIR, IgG2a/c: EDYNSTLR, IgG3: EAQYNSTFR.