Table 2. Available Level 2 vectors.
Level 1 modules used for assembly | Corresponding Level 2 vectors (HR1/HR2) | Auxotrophic markers for selection |
---|---|---|
A, B | pL2_AB_hc/pl2_AB_lc (A0_C0) | URA3, LEU2 |
A, B, C | pL2_AC_hc/pL2_AC_lc (A0_D0) | URA3, LEU2, HIS3 |
A, B, C, D | pL2_AD_hc/pL2_AD_lc (A0_E0) | URA3, LEU2, HIS3, LYS2 |
A, B, C, D, E | pL2_AE_hc/pl2_AE_lc (A0_F0) | URA3, LEU2, HIS3, LYS2, TRP1 |
Depending on the number of Level 1 modules to assemble, different Level 2 vectors must be used. Each vector is available in high- and low-copy format (hc/lc). Depending on the assembled Level 1 modules, the resulting Level 2 construct can be selected with up to five auxotrophic markers.