Table 3. Efficiency of Level 1 and 2 assemblies during the 25-gene demo assembly.
Assembly step | Positive in colony PCR | Confirmed by restriction analysis | Confirmed by sequencing |
---|---|---|---|
1A | 24/24 | 4/4 | 2/2 |
1B | 24/24 | 4/4 | 2/2 |
1C | 24/24 | 4/4 | 2/2 |
1D | 24/24 | 4/4 | 2/2 |
1E | 15/24 | 4/4 | 2/2 |
2 | 24/24 | 1/1 | 1/1 |