Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Assembly step | Positive in colony PCR | Confirmed by restriction analysis | Confirmed by sequencing |
|---|---|---|---|
| 1A | 24/24 | 4/4 | 2/2 |
| 1B | 24/24 | 4/4 | 2/2 |
| 1C | 24/24 | 4/4 | 2/2 |
| 1D | 24/24 | 4/4 | 2/2 |
| 1E | 15/24 | 4/4 | 2/2 |
| 2 | 24/24 | 1/1 | 1/1 |
