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Single-nucleotide competition assay comparing the primer-extension efficiency of tCTP versus tCfTP. Competition assays were performed using triphosphate solutions that contained defined ratios of tCTP and tCfTP ranging from 0 to 100% tCfTP. Primer-extension reactions were performed on primer–template complexes that were either all-DNA (A) or used a DNA/TNA chimeric primer that contained 5 TNA residues at the 3΄ end (B). Each dataset represents the average of two independent trials.
