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. 2017 Apr 8;45(10):5887–5900. doi: 10.1093/nar/gkx221

Figure 2.

Figure 2.

SWI/SNF regulates recruitment of MRX to a MAT DSB. (A) Asynchronous WT (n = 7) and snf5Δ (n = 6) cells were harvested at 1 h intervals after addition of galactose to induce a MAT DSB. Recruitment of Mre11 0.1 kb to the right of the MAT DSB was monitored by ChIP (left) and DNA end resection was simultaneously monitored (right). (B) Asynchronous WT (n = 3) and snf5Δ (n = 3) cells were harvested after addition of galactose. Recruitment of Ku70 (left) and DNA end resection (right) were monitored as in A. (C) Asynchronous ku70Δ (n = 5) and snf5Δku70Δ (n = 3) cells were harvested after addition of galactose. Recruitment of Mre11 (left) and DNA end resection (right) were monitored as in A. WT (solid line) and snf5Δ (dashed line) Mre11 ChIP recruitment data from A are overlaid on the graph. Error bars denote one standard deviation. Statistical differences between strains at time points were assessed by two-way ANOVA with Holm–Sidak post-hoc analysis. *P < 0.05, **P < 0.01, ***P < 0.001.