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. 2017 Apr 8;45(10):5887–5900. doi: 10.1093/nar/gkx221

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Long-range resection is delayed in snf5Δ cells and relies upon Exo1. Asynchronous WT, snf5Δ, exo1Δ, snf5Δexo1Δ, sgs1Δ and snf5Δsgs1Δ cells (n = 3) were harvested at 2 h intervals after addition of galactose to induce a MAT DSB. Resection was monitored by qPCR with primers annealing either 0.1 kb (A) or 12.0 kb (B) to the right of the MAT DSB. (C) The time for 25% resection to occur (0.75 fraction intact) at positions to the right of the MAT DSB. (D) The time for 25% resection to occur immediately adjacent to the MAT was designated as the time to resection initiation, and (E) resection rates were calculated by determining the slopes of the graphs by linear regression analysis. Error bars denote one standard deviation. Statistical comparisons between strains were assessed by Student's t-test. *P < 0.05, **P < 0.01, ***P < 0.001.