Figure 2.

Complementation of SUB1 deletion in top1Δ and top1Y727F backgrounds. (A) Protein sequence alignment between human PC4 (hPC4) and S. cerevisiae Sub1 (ySub1). Identical and conservative residues are indicated by bullets (•) and plus signs (+), respectively. Only the first 116 a.a. of Sub1 (292 a.a.) are shown. (B–E) All graphs are showing the rates of recombination (×10⁻⁸). Error bars indicate 95% confidence intervals. Prior to fluctuation analysis to determine the rates of recombination, indicated yeast strains were transformed with empty vector (+Vec), Sub1-expression plasmid (+SUB1), or PC4-expression vector (+PC4).