Figure 2.
sae processing requires an active RNase Y. Wild-type, rny mutant, complemented strain with truncated RNase Y lacking its active site (ΔAS) and complemented strains with RNase Y with point mutations in His367 and Asp368 that constitute the highly conserved HD motif (367AA), all carrying the sae-gfp constructs indicated in the figure, were grown to exponential phase. RNA was harvested and hybridized to DIG-labeled DNA probes specific for gfp or rny. As a loading control, 16S rRNA was detected in the ethidium bromide-stained gel, as shown at the bottom of the figure.