Figure 6.

Double strand structure downstream of CS determines sae processing. (A) Schematic representation of construct pCG484 carrying a deletion in the Rrs counterpart (Rrs*, indicated by a white rectangle). The RNAs detected in the northern blot analysis are shown below each construct. (B) Northern blot analyses to examine sae processing in strains carrying pCG384. Newman wild-type and rny mutant strains carrying the different constructs were grown to the exponential phase (strains carrying pCG212 and pCG392 were included in the analysis as controls). RNA was harvested and hybridized to DIG-labeled DNA probes specific for gfp and rny. As a loading control, 16S rRNA was detected in the ethidium bromide-stained gel, as shown at the bottom of the panel. (C) Schematic representation of construct pCG599 (reference construct in replicative vector, equivalent to pCG212), pCG616 (carrying point mutation in CS) and pCG618 (carrying point mutations which affect Rrs secondary structure). The RNAs detected in the northern blot analysis are shown below each construct. (D) Northern blot analyses to examine sae processing in strains carrying pCG599, pCG616 und pCG618. Newman wild-type carrying the different constructs was grown to exponential phase. RNA was harvested and hybridized to DIG-labeled DNA probes specific for gfp and rny. As a loading control, 16S rRNA was detected in the ethidium bromide-stained gel, as shown at the bottom of the panel.