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. 2017 Apr 27;45(10):5980–5994. doi: 10.1093/nar/gkx296

Figure 7.

Figure 7.

Interference of RNase J1 with RNase Y activity. (A) Schematic representation of construct pCG601, pCG600 and pCG620, which are the equivalent in replicative plasmid of construct pCG392, pCG484 and pCG223, respectively. (B) Northern blot analyses to examine sae processing in strains carrying pCG600, pCG601 and pCG620. PR wild-type and rnjA mutant strains carrying the different construct were grown to the exponential phase (strains carrying the reference construct pCG599 were included in the analysis as controls). RNA was then harvested and hybridized to DIG-labeled DNA probes specific for gfp, rny and rnjA. As a loading control, 16S rRNA was detected in the ethidium bromide-stained gel, as shown at the bottom of the panel. (C) Northern blot analyses to detect rny transcript in PR strains. Newman wild-type and rny mutant, PR wild-type and rnjA mutant strains were grown to exponential phase. RNA was harvested and hybridized to DIG-labeled DNA probes specific for rnjA, rny and rny 5΄. As a loading control, 16S rRNA was detected in the ethidium bromide-stained gel, as shown at the bottom of the panel.