|
saeP deletion mutant
|
|
ISP479C |
Kpnsae-for |
CGGGGTACCATACTACAGTTTTACATT |
|
|
Kpn-ORF4-rev |
ACCTCGGTACCCTGTTCTTACGACCTCTAAAG |
|
|
HybridORF4a-rechts |
TAAAAGTTCGCTAGATAGGGGTCCCGCGTATGATTTCACAGCC |
|
|
Hybridsae-links |
TCCAATTCTCGTTTTCATACCTCGGAGCTAACTCCTCATTTCTTCAATTT |
|
Newman29 |
kanR-for |
CCGAGGTATGAAAACGAGAATTGG |
|
|
kanR-rev |
GGGACCCCTATCTAGCGAACTTT |
|
sae–gfp fusion in integrative plasmid (pCG188)
|
|
|
Eco-sae-for |
GCGTGAATTCTTATTGTGGCAAAAGGTTT |
|
|
Eco-sae1283rev |
CGTGAATTCTGACGTCGTATGTGCAACTA |
|
|
Eco-sae1014rev |
GGGAATTCTTATGTGAACAGGAAGTGTTT |
| control PCRs |
|
gfp-rev-all |
GGTATCACCTTCAAACTTGACTT |
| pCG213 |
pcwsae19 |
DelP2for |
TCAATATATATACCATAAGATTGC |
|
|
DelP2rev |
GCAATCTTATGGTATATATATTGA |
| pCG218 |
pcwsae19 |
DelST-orf4for |
AGAGCACATAAGAAACACTTCCT |
|
|
DelST-orf4rev |
AGGAAGTGTTTCTTATGTGCTCT |
| pCG219 |
pcwsae19 |
DelST5for |
TCAATGGAAAGCATATATACAACT |
|
|
DelST5rev |
AGTTGTATATATGCTTTCCATTGA |
| pCG223 |
pcwsae19 |
DelP2longfor |
TCAATGGAAAGCAAACACTTCCT |
|
|
DelP2longrev |
AGGAAGTGTTTGCTTTCCATTGA |
| pCG379 |
pCG218 |
DelST5rev |
AGTTGTATATATGCTTTCCATTGA |
|
|
DelST5for |
TCAATGGAAAGCATATATACAACT |
| pCG301 |
pcwsae19 |
hybridsaePdelrev |
CTTTCCATTGAGTAACCTTGATCTTGTGA |
|
|
hybridsaePdelfor |
CACAAGATCAAGGTTACTCAATGGAAAGC |
| pCG392 |
pcwsae19 |
hybridpCG392rev |
TCAAGCTCTAAAAAAATTTAGATTTAATAGTTGTATATAT |
|
|
hybridpCG392for |
ATATATACAACTATTAAATCTAAATTTTTTTAGAGCTTGAT |
| pCG394 |
pcwsae19 |
hybridpCG394rev |
TGTGCTCTGCAATCTTATGGATTGAAAAAAGGAAAGTATG |
|
|
hybridpCG394for |
CATACTTTCCTTTTTTCAATCCATAAGATTGCAGAGCACA |
| pCG484 |
pcwsae19 |
Hybrid-Rrs-Counter-rev |
CGATTTGTAGTGTTATGTGA |
|
|
Hybrid-Rrs-Counter-for |
TCACATAACACTACAAATCGTTTATATAAATTACACACAAT |
| pCG589 |
pCG212 |
Q5SDMpCG212R |
ATATATTGAAAAAAGGAAAGTATGATTTC |
|
|
Q5SDMpCG212F |
ATACAACTATCAAATCCCATAAGATTG |
|
sae–gfp fusion in replicative plasmid (pCG246)
|
| pCG599 |
pCG212 |
Gibson-saeconstr-for |
ATTTAGAATAGGCGCGCCTGAATTCTTATTGTGGCAAAAGGTTTATAAATTTTAATAC |
|
|
Gibson-saeconstr-rev |
ATCCCCGGGTACCGAGCTCGAATTCTTACAAACAAAAAGCGGATTAC |
| pCG600 |
pCG484 |
Gibson-saeconstr-for |
ATTTAGAATAGGCGCGCCTGAATTCTTATTGTGGCAAAAGGTTTATAAATTTTAATAC |
|
|
Gibson-saeconstr-rev |
ATCCCCGGGTACCGAGCTCGAATTCTTACAAACAAAAAGCGGATTAC |
| pCG601 |
pCG392 |
Gibson-saeconstr-for |
ATTTAGAATAGGCGCGCCTGAATTCTTATTGTGGCAAAAGGTTTATAAATTTTAATAC |
|
|
Gibson-saeconstr-rev |
ATCCCCGGGTACCGAGCTCGAATTCTTACAAACAAAAAGCGGATTAC |
| pCG616 |
pCG589 |
Gibson-saeconstr-for |
ATTTAGAATAGGCGCGCCTGAATTCTTATTGTGGCAAAAGGTTTATAAATTTTAATAC |
|
|
Gibson-saeconstr-rev |
ATCCCCGGGTACCGAGCTCGAATTCTTACAAACAAAAAGCGGATTAC |
| pCG618 |
pCG599 |
Q5SDMpCG618F |
AAGACCGCAGAGCACATAAGTAAATTTTTTTAG |
|
|
Q5SDMpCG618R |
AGGGGAGTTAATAGTTGTATATATATTGAAAAAAGG |
| pCG620 |
pCG223 |
Gibson-saeconstr-for |
ATTTAGAATAGGCGCGCCTGAATTCTTATTGTGGCAAAAGGTTTATAAATTTTAATAC |
|
|
Gibson-saeconstr-rev |
ATCCCCGGGTACCGAGCTCGAATTCTTACAAACAAAAAGCGGATTAC |
|
RNase Y Complementation
|
| pCG322 |
RN6390 |
RecA-dig-for |
GTCAAGGTAAGGAAAATGTT |
|
|
hybrid-Rny-delKD-rev |
TGCACCTGGACGAGCTACATTTTGACCGT |
|
|
hybrid-Rny-delKD-for |
ACGGTCAAAATGTAGCTCGTCCAGGTGCA |
|
|
SAV1287-rev |
AACAATTTGTTGCAATTG |
|
|
BamHI-RNY-for |
CCGGATCCGTTAAACTTAGCAAATATCCT |
|
|
EcoRI-RNY-rev |
CGAATTCCTCAACTTAGAAATAAATCCTA |
| pCG596 |
pCG296 |
Q5SDMpCG296R |
GCTCGTTTCGCTAATGTC |
|
|
Q5SDMpCG296F |
TGGACTTTTAGCTGCTGTTGGTAAAGCAATTGATC |
|
Race
|
|
|
RNA 5΄ adapter |
CTAGTACTCCGGTATTGCGGTACCCTTGTACGCCTGTTTTATA |
|
|
gfp-rev1 |
TCTTTTGTTTGTCTGCCAT |
|
|
Race 2 |
Race 2 GTATTGCGGTACCCTTGT |
|
|
gfp-rev-all |
GGTATCACCTTCAAACTTGACTT |
|
DIG probes
|
|
gfp
|
pCG188 |
gfp-dig-for |
CACTTGTCACTACTTTCGGTT |
|
|
gfp-dig-rev |
TCTCTCTTTTCGTTGGGAT |
|
saeP
|
RN6390 |
uorf4358 |
TATTATTTGCCTTCATTTTA |
|
|
lorf4616 |
ACCTTTTGATGATTTGTAGTTAG |
|
rny
|
RN6390 |
SAV1286dig-for |
TTATTAGAGAAGCAGGTGAACA |
|
|
SAV1286dig-rev |
TCTTCAGGAGATACAATCACTC |
|
rny 5 prime
|
RN6390 |
RNY-dig-for2 |
TTCATATAAAGAGCAAACCC |
|
|
RNY-dig-rev2 |
TTTTGATATTGTCAGCTTCT |
|
rnjA
|
RN6390 |
sav1089digfor |
CACCGATACCACTACCAT |
|
|
sav1089digrev |
ACCTGAAGATACCGTTGT |
|
RT-qPCR
|
|
saeR standard
|
Newman |
T7sae |
TAATACGACTCACTATAGGGAGAAACTGCCAAAACACAAGA |
|
|
saeR2 |
CCATTATCGGCTCCTTTCA |
|
saeP standard
|
Newman |
T7-ORF4 |
TAATACGACTCACTATAGGGAGACAAATTGAAGAAATGAGGAGTTA |
|
|
lorf4616 |
ACCTTTTGATGATTTGTAGTTAG |
|
saeR
|
|
saeR4 |
TAGTCATATCCCCAAACTT |
|
|
saeU4 |
CCATTTACGCCTTAACTTTA |
|
saeP
|
|
uorf4358 |
TATTATTTGCCTTCATTTTA |
|
|
lorf4616 |
ACCTTTTGATGATTTGTAGTTAG |
|
Protein purification
|
| pCG249 |
Newman |
Xho-rny-pETfor |
GGGGCTCGAGCGAAATTTGTTGCTTCAAAAG |
|
|
Bam-rny-pETrev |
GGGGGGATCCTTATTTCGCATATTCTACTGCT |
|
Transcripts for RNase Y in vitro cleavage assay
|
|
pCG212 and pCG484 |
T7saeorf4u |
TAATACGACTCACTATAGGGAGACAAATTGAAGAAATGAGGAGTTA |
|
|
LCgfpmut3.1rev1 |
TCTTTTGTTTGTCTGCCAT |
