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. 2017 Feb 10;45(10):e88. doi: 10.1093/nar/gkx093

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — AmpSeq-SSR: its procedure, characteristics and comparison with capillary electrophoresis (CE). (A) A sketch of AmpSeq-SSR procedure applied to genotyping 3105 SSRs within 24 h. (B) Validation of an example SSR genotype in variety F using Sanger sequencing. (C, D and E) Profiles of AMPL1118228 (C) and AMPL1122663 (D) in variety H by CE and the comparisons of their genotypes with the genotypes from AmpSeq-SSR (E). (F) Amplicons with 40–70% GC contents have relatively uniform coverages. (G) The length distributions of all of the target SSRs on the reference genome. (H) The length distributions of the motifs of the target SSRs on the reference genome.