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. 2017 Feb 10;45(10):e88. doi: 10.1093/nar/gkx093

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Mapping gene Xa21 by AmpSeq-SSR. (A) SSR21 is the only SSR with differential genotypes between every pair of the three nearly isogenic lines (NILs). (B) Identical genotypes of (CGC)7 among the receptor parental varieties of the NILs. (C) No genotype difference between NILs can be detected by PAGE electrophoretogram. The molecular weight standards are marked on the left of the electrophoretogram. (D) The sequences of SSR21 amplicons in the three pairs of NILs. Letters in yellow background are the sequences of SSR21. The nucleotide substitution variations are marked by black arrow. Numbers of sequencing reads are listed on the left of the sequences.