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. 2017 May 2;45(10):5653–5665. doi: 10.1093/nar/gkx331

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — In vitro experimental validation of rs11540855-miR-4707-3p-ABHD8-AXIN1. (A) Graphic representation of genetic effect on miR-4707-3p-ABHD8-AXIN1, circle: ceD and ceT; triangle: miRNA; suppression line with label: the miRNA-ceD regulation, G for gain-of-function mutation; other suppression lines: the miRNA-ceT regulation; arrow: ceD activate ceTs in gain-of-function situation ( and ). (B) Fold change of luciferase activity ratio for ABHD8 A-allele or ABHD8 G-allele (Firefly luciferase) and the internal control (Renilla luciferase) in response to miR-4707-3p agomir, n = 6 experiments; (C) Fold change of relative ABHD8 A-allele or ABHD8 G-allele mRNA expression level (with GAPDH) in response to miR-4707-3p agomir, n = 6 experiments; (D) A representative immunoblot and fold change of MCF116 native AXIN1 expression in response to miR-4707-3p agomir, n = 5 experiments; (E) A representative immunoblot and fold change of AXIN1 expression in MCF116 cells stably expressing the two ABHD8 alleles in response to miR-4707-3p agomir, n = 5 experiments; (F) Relative expression level of miR-4707-3p, ABHD8 and AXIN1, as a function of miR-4707-3p agomir dosage (range: 0, 5, 10, 15, 20 pmol/well), n = 6 experiments.

Inline graphic — In vitro experimental validation of rs11540855-miR-4707-3p-ABHD8-AXIN1. (A) Graphic representation of genetic effect on miR-4707-3p-ABHD8-AXIN1, circle: ceD and ceT; triangle: miRNA; suppression line with label: the miRNA-ceD regulation, G for gain-of-function mutation; other suppression lines: the miRNA-ceT regulation; arrow: ceD activate ceTs in gain-of-function situation ( and ). (B) Fold change of luciferase activity ratio for ABHD8 A-allele or ABHD8 G-allele (Firefly luciferase) and the internal control (Renilla luciferase) in response to miR-4707-3p agomir, n = 6 experiments; (C) Fold change of relative ABHD8 A-allele or ABHD8 G-allele mRNA expression level (with GAPDH) in response to miR-4707-3p agomir, n = 6 experiments; (D) A representative immunoblot and fold change of MCF116 native AXIN1 expression in response to miR-4707-3p agomir, n = 5 experiments; (E) A representative immunoblot and fold change of AXIN1 expression in MCF116 cells stably expressing the two ABHD8 alleles in response to miR-4707-3p agomir, n = 5 experiments; (F) Relative expression level of miR-4707-3p, ABHD8 and AXIN1, as a function of miR-4707-3p agomir dosage (range: 0, 5, 10, 15, 20 pmol/well), n = 6 experiments.