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CK2-phosphorylated XRCC4–LigIV can bind PNKP–DNA substrate complexes. EMSA experiments were carried out using the FAM-labeled DNA constructs illustrated to the left in the absence of XRCC4–LigIV (left panels), with 2 μM XRCC4–LigIV (center panels), or with 2 μM pXRCC4–LigIV (right panels). The PNKP concentrations increased from left to right in each gel. The PNKP concentrations are: 0, 40, 80, 200, 400, 800, 2000 nM.
