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. 2017 Feb 16;45(10):e89. doi: 10.1093/nar/gkx113

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Comparison of transfection methods and transposon sizes for in vivo transposition. (A) In vivo transposition efficiency after electroporation or chemical transfection. In vivo transposition efficiency is the number of resistant colonies per microgram of the donor plasmid divided by the efficiency of transfection of a standard plasmid (CFU/μg). (B) Agarose gel of the restriction analysis to confirm the correct size of each plasmid donor of transposon assembled by PaperClip. (C) Correlation of the transposition efficiency with the transposon size after Mos1 transposition. For panels A and C two technical repeats were performed for two biological repeats.