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. 2017 Feb 15;45(10):e90. doi: 10.1093/nar/gkx117

Figure 2.

Figure 2.

(A) The fluorescence intensity responses of BM-1 probe toward PM-1 target and 1-MM-1 target. (B) Illustration of the reaction pathways of BM-1 probe toward PM-1 target and 1-MM-1 target with and without the assistance of polymerase extension. (C) The fluorescence response curves of BM-1 probe toward PM-1 target and 1-MM-1 target in a concurrent reaction of branch migration and polymerase elongation. (D) Gel electrophoresis results of the branch migration reaction products shown in (A). Lane 1: BM-1 probe + PM-1 target. Lane 2: BM-1 probe + 1-MM-1 target. (E) The discrimination factors (DF) of BM probe toward different types of mismatches. The data were obtained based on the rate of fluorescence increase of the BM probe with the assistance of polymerase elongation. The six types of mismatches indicated by the blue bracket can cover all the 12 types of mismatches as the real DNA samples come as double stranded.