Figure 5.

(A) The fluorescence intensity responses of BM-4 probe toward BRAF mutant-type targets at different abundance levels. Inset shows the increase rates of fluorescence intensity (FL) for the BRAF mutant-type targets at different abundance levels. All the experiments were repeated for three times, and standard deviations were shown in error bars. (B) Detection of BRAF V600E mutation in circulating free DNA (cfDNA) extracted from the serum of a thyroid cancer patient. Serum samples obtained from a healthy person and a liver cancer patient were also measured as controls.