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. 2017 Mar 28;45(10):5863–5876. doi: 10.1093/nar/gkx209

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Excision of uracil from different structural contexts. Uracil excision assays with DNA oligomers (∼0.1 pmol each) containing a different number of nucleotides on either side of uracil (A) or with the hairpin DNA oligomers containing uracil in different positions in tetraloop (B) were carried out at 37°C as described in Materials and Methods. (A) Reactions were carried out with 1 μg of either protein (∼40 pmol EcoUng or ∼32 pmol BdiUng monomer) for 30 min. More than one product band is observed in some reactions because incomplete alkaline cleavage of AP site (first reaction leaves a semialdehyde residue on 3΄ end of 5΄ product and the second reaction removes it, producing a 3΄ phosphate on the 5΄ product; products are marked with asterisks). (B) Reactions were performed for 30 min with 100 pg (4 fmol) and 500 ng (∼16 pmol monomer) of EcoUng and BdiUng, respectively. Reaction products were resolved on 8 M urea PAGE (15%).