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Activities of BdiUng A59Y mutant. The excision assays were carried out with oligomers (∼0.1 pmol each) containing different lesions (uracil in ssU9, xanthine in ssX9 and 5-hydroxymethyluracil in HmU) using BdiUng and BdiUng A59Y mutant (each 1 μg, ∼32 pmol monomer) at 37°C for 30 min as described in Materials and Methods. Products were resolved on 8 M urea PAGE (15%).
