FIG 4 .

Accumulation of VIT proteins leads to intestinal degeneration. (A) Photomicrographs of the parental strain SJ4005 and mutant animals grown on E. coli HT115 containing empty vector at 20°C until they were 1-day-old adults before images were acquired. (B) Survival of SJ4005 and mutant animals on P. aeruginosa PA14 at 25°C after treatment with control RNAi. The animals were grown on E. coli HT115 containing empty vector at 20°C until they were 1-day-old adults before transferring to P. aeruginosa PA14 at 25°C. P values comparing the values for mutant animals to the values for SJ4005 animals are as follows: P < 0.001 for vit-1(ac2), P < 0.001 for vit-2(ac3), P < 0.001 for vit-4(ac4), P < 0.001 for vit-5(ac5), and P < 0.001 for vit-6(ac6). (C) Photomicrographs of SJ4005 and mutant animals grown on E. coli HT115 containing vector for RNAi against ire-1 at 20°C until they were 1-day-old adults before images were acquired. (D) Survival of SJ4005 and mutant animals on P. aeruginosa PA14 at 25°C after treatment with ire-1 RNAi. The animals were grown on E. coli HT115 containing vector for RNAi against ire-1 at 20°C until they were 1-day-old adults before being transferred to P. aeruginosa PA14 at 25°C. The P values for all mutant animals relative to the values for SJ4005 animals are P < 0.001. (E) Photomicrographs of SJ4005 and mutant animals grown on E. coli HT115 containing vector for RNAi against xbp-1 at 20°C until they were 1-day-old adults before images were acquired. (F) Survival of SJ4005 and mutant animals on P. aeruginosa PA14 at 25°C after xbp-1 RNAi. The animals were grown on E. coli HT115 containing vector for RNAi against xbp-1 at 20°C until they were 1-day-old adults before being transferred to P. aeruginosa PA14 at 25°C. P values for all mutant animals relative to SJ4005 animals are P < 0.001. Survival curves are representative assays for three independent experiments. n = 60 to 100.