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Expression and purification of LgBSH. SDS–PAGE analysis of LgBSH (A). Lane1: Marker (PageRulerTM Prestained Protein Ladder, 10–180 kDa), Lane2: cell lysate of Escherichia coli BL21 wild type, Lane3: cell lysate of E. coli BL21 expressing LgBSH, Lane4: purified LgBSH. Western blotting analysis of LgBSH using anti-His antibody (B). Lane1: cell lysate of E. coli BL21 wild type, Lane2: cell lysate of E. coli BL21 expressing LgBSH, Lane3: purified LgBSH.
