Table 1.
Specificity of the multiplex real-time PCR primers for different bacterial strains.
| Bacterial stains | Sourcea | Multiplex real-time PCR results |
||
|---|---|---|---|---|
| nuc | hlyA | orgC | ||
| Staphylococcus aureus | ATCC 25923 | + | - | - |
| CICC 21648 | + | - | - | |
| CICC 10786 | + | - | - | |
| CMCC 41002 | + | - | - | |
| CMCC 26003 | + | - | - | |
| CGMCC 1.89 | + | - | - | |
| CGMCC 1.128 | + | - | - | |
| Listeria monocytogenes | ATCC 19115 | - | + | - |
| ATCC 19114 | - | + | - | |
| ATCC 19112 | - | + | - | |
| ATCC 19116 | - | + | - | |
| ATCC 19118 | - | + | - | |
| CMCC 54002 | - | + | - | |
| Salmonella spp. | ||||
| Salmonella typhimurium | ATCC 14028 | - | - | + |
| Salmonella typhimurium | CMCC 50362 | - | - | + |
| Salmonella enterica | CMCC 50041 | - | - | + |
| Salmonella Paratyphi B | CMCC 50094 | - | - | + |
| Other stains | ||||
| Campylobacter jejuni | ATCC 33560 | - | - | - |
| Vibrio parahaemolyticus | ATCC 33847 | - | - | - |
| Vibrio parahaemolyticus | ATCC 17802 | - | - | - |
| Vibrio vulnificus | ATCC 27562 | - | - | - |
| Escherichia coli O157:H7 | ATCC 43889 | - | - | - |
| Escherichia coli O157:H7 | CICC 10907 | - | - | - |
| Escherichia coli | CMCC 44568 | - | - | - |
| Enterococcus faecalis | CGMCC 1.2135 | - | - | - |
| Listeria welshimeri | ATCC 43548 | - | - | - |
| Listeria welshimeri | ATCC 43550 | - | - | - |
| Listeria innocua | ATCC 33091 | - | - | - |
| Bacillus subtilis | CGMCC 1.4255 | - | - | - |
| Bacillus cereus | CMCC 70331 | - | - | - |
| Shigella boydii | CGMCC 1.10618 | - | - | - |
| Shigella flexneri | CGMCC 1.10599 | - | - | - |
aATCC, American Type Culture Collection, United States; CMCC, China Medical Culture Collection, China; CICC, China Center of Industrial Culture Collection, China; CGMCC, China General Microbiological Culture Collection Center, China.